Zhongyi Yang, Hanying Hu, Yuling Zou, Wenbluo Luo, Lin Xie and Zhipeng You* Pages 372 - 378 ( 7 )
Background: Diabetic retinopathy (DR) is a common complication of diabetes. This study investigated the effect of miR-7 in the regulation of cell proliferation via the HoxB3 gene and PI3K/AKT/mTOR signaling pathways in DR. <p></p> Methods: Human retinal pigment epithelial cell line (ARPE-19) cultured in normal medium (Control) and high glucose medium (25mM glucose, HG) was transfected with mimics NC (HG+ mimics NC), miR-7 mimics (HG+miR-7 mimics), inhibitor NC (HG+ inhibitor NC), and miR-inhibitor (HG+miR-7 inhibitor). The cells were assayed for viability, apoptosis, and expression of genes. <p></p> Results: HG reduced cell viability and increased apoptosis. However, miR-7 mimics reduced the apoptosis. PCR results showed that miR-7 was significantly upregulated after transfection with miR-7 mimics. The expression of Hoxb3, mTOR, p-PI3K, and p- AKT was significantly downregulated at mRNA and protein levels after miR-7 mimics transfection, while no difference was observed for PI3K and AKT expression. <p></p> Conclusion: Our findings demonstrate that miR-7 regulates the growth of retinal epithelial cells through various pathways and is a potential therapeutic target for the prevention and treatment of diabetic retinopathy.
miR-7, HoxB3, DR, PI3K/AKT/mTOR signaling, retinopathy, celll proliferation.
Department of Ophthalmology, The Second Affiliated Hospital of Nanchang University, Nanchang, Department of Ophthalmology, The Second Affiliated Hospital of Nanchang University, Nanchang, Department of Ophthalmology, The Second Affiliated Hospital of Nanchang University, Nanchang, Department of Ophthalmology, The Second Affiliated Hospital of Nanchang University, Nanchang, Department of Ophthalmology, The Second Affiliated Hospital of Nanchang University, Nanchang, Department of Ophthalmology, The Second Affiliated Hospital of Nanchang University, Nanchang