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Direct SARS-CoV-2 Detection System Utilizing Simple-to-Use Capillary Gel Electrophoresis Sample-to-Result

[ Vol. 24 , Issue. 1 ]


Neo Yang, Meya Kuo, Jerry Liu, Varoujan Amirkhanian* and Eric Tsai   Pages 145 - 150 ( 6 )


<P>Aim: We present a Direct SARS-CoV-2 Detection System that achieves sample-to-results in less than two hours in three simple steps. <P> Methods: The Detection System includes Direct one-step Reverse Transcription PCR (RT-PCR) reagents (Qexp-MDx kit), a portable thermal cycler (Qampmini) with a preprogrammed chip and a simple-to-use Capillary Gel Electrophoresis system (Qsep Series Bio-Fragment Analyzer) with high fluorescence detection sensitivity to solve the problems associated with traditional real-time PCR (qPCR) systems which produces high false positive results. <P> Results: The proposed simple-to-use detection platform can provide high detection sensitivity (identify less than 20 copies) and fast results (less than 120 minutes), which would be suitable for field testing applications. <P> Conclusion: Our high detection sensitivity platform provides fast and accurate results in 120 minutes without doing DNA/RNA extraction.</P>


Polymerase Chain Reaction (PCR), Reverse Transcription PCR (RT-PCR), gel electrophoresis, Capillary Electrophoresis (CE), fragment analyzer, pandemic, SARS-CoV-2, Coronavirus, COVID-19.


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