N. Guerrero, M. M. Meynard, J. Borgonovo, K. Palma, M. L. Concha and C. Hetz* Pages 13 - 23 ( 11 )
More than thirty years have passed since the discovery of the prion protein (PrP) and its causative role in transmissible spongiform encephalopathy. Since a combination of both gain- and loss-of-function mechanisms may underlay prion pathogenesis, understanding the physiological role of PrP may give important clues about disease mechanisms. Historically, the primary strategy for prion research has involved the use of human tissue, cell cultures and mammalian animal models. Nevertheless, experimental difficulties of in vivo studies and controversial observations obtained in these systems have stimulated the search for alternative animal models. PrPC is highly conserved in mammals, and PrPC-related orthologs are expressed in zebrafish, a vertebrate model organism suitable to study the mechanisms associated with human diseases. Invertebrate models, as they do not express PrPC have served to investigate the neurotoxic mechanisms of mammalian PrP. Here we overview most recent advances in the study of PrP function in normal and pathogenic conditions based on non-mammalian studies, highlighting the contribution of zebrafish, fly and worms to our current understanding of PrP biology.
Prion-related disorders, PrPC, physiology, neurotoxicity, mammals, zebrafish, Drosophila melanogaster, Caenorhabditis elegans.
Anatomy and Developmental Biology Program, Institute of Biomedical Sciences, Faculty of Medicine, Universidad de Chile, PO Box 70031, Santiago, Biomedical Neuroscience Institute, Independencia 1027, Santiago, Center for Geroscience, Brain Health and Metabolism, Santiago, Center for Geroscience, Brain Health and Metabolism, Santiago, Institute of Biomedical Sciences (Sector A, Second Floor), Universidad de Chile, Independencia 1027, Santiago, P.O. Box 70031, Institute of Biomedical Sciences (Sector B, Second Floor), Universidad de Chile, Independencia 1027, Santiago, P.O. Box 70086