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Crocin Protects Human Umbilical Vein Endothelial Cells from High Glucose-Induced Injury Via Inhibiting the Endoplasmic Reticulum Stress Response

[ Vol. 18 , Issue. 3 ]

Author(s):

H. Zhang, S. Wu, Y. Yang, R. Su, J. Wen, X. Ke* and W. Chen*   Pages 166 - 177 ( 12 )

Abstract:


Background: Endothelial dysfunction plays a key role in diabetic atherosclerosis. High glucose (HG) is considered a stimulator in the development of diabetic atherosclerosis. The endoplasmic reticulum (ER) stress response is involved in HG-induced vascular injury. Crocin has antioxidative and antiapoptotic properties.

Objective: The current study was to evaluate whether crocin can protect human umbilical vein endothelial cells (HUVECs) from HG-induced injury and explored the associated mechanism.

Methods: HUVECs were treated with 33 mmol/L glucose as the HG condition. The endothelial protective effects of crocin were evaluated by comparison with the control groups.

Results: The exposure of HUVECs to HG for 24 h remarkably induced the ER stress response and a sequence of injuries, as demonstrated an increase in the apoptotic cell number, the reactive oxygen species level and inflammatory cytokine generation, as well as a decline in vascular endothelial growth factor A expression. These changes were markedly alleviated by pretreating the HUVECs with either crocin or 4-phenylbutyrate (ER stress inhibitor) before exposure to HG.

Conclusion: Crocin exerted antioxidative, antiapoptotic, anti-inflammatory and proangiogenic effects in the HG-induced HUVEC injury model, which were probably mediated by a favorable modification of ER stress that requires further investigation.

Keywords:

Endothelial cells, crocin, protective effects, oxidation, inflammation, apoptosis.

Affiliation:

Department of Cardiology, The Eight Affiliated Hospital, Sun Yat-sen University, Shenzhen, Department of Cardiology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Department of Cardiology, Huangpu Division of The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Department of Cardiology, Shenzhen Sun Yat-sen Cardiovascular Hospital, Shenzhen, Department of Intensive Unit, Shenzhen Sun Yat-sen Cardiovascular Hospital, Shenzhen, Department of Cardiology, Shenzhen Sun Yat-sen Cardiovascular Hospital, Shenzhen, Department of Intensive Unit, Shenzhen Sun Yat-sen Cardiovascular Hospital, Shenzhen



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