Submit Manuscript  

Article Details


MiR-19b Functions as a Potential Protector in Experimental Autoimmune Encephalomyelitis

[ Vol. 18 , Issue. 5 ]

Author(s):

B. Hu, Z.L. Hu, Q.M. Zeng, B. Xiao and H. Yang*   Pages 312 - 321 ( 10 )

Abstract:


Background: MicroRNA-19b (miR-19b) is essential in determining oligodendroglia proliferation. Phosphatase and tensin homologue on chromosome 10 (PTEN) is considered the target of miR-19b and participates in oligodendrocyte differentiation and proliferation.

Methods: Murine EAE was induced by myelin oligodendrocyte glycoprotein (MOG35– 55). For EAE reversal, artificially synthesized agomiR-19b was intravenous injected after immunization.

Results: We found that the expression of miR-19b is significantly reduced in an experimental autoimmune encephalomyelitis (EAE) mouse model. This downregulation, which is associated with the neurological scores, can be dramatically ameliorated by agomiR-19b. Our results show that agomiR-19b increases the expression of myelin basic protein (MBP) and cyclic nucleotide phosphodiesterase (CNP), which are regularly utilized as molecular markers of oligodendrocytes. Furthermore, our study also revealed that miR-19b probably affects the expression of PTEN in the EAE model.

Conclusion: These results indicate that the restoration of miR-19b probably exerts its therapeutic effect by affecting PTEN in the pathogenesis of EAE.

Keywords:

MiR-19b, experimental autoimmune encephalomyelitis, oligodendrocyte, PTEN, agomiR-19b, MBP, CNP.

Affiliation:

Department of Neurology, Xiangya Hospital of Central South University, Changsha, 410008, Department of Anesthesiology, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, Department of Neurology, Xiangya Hospital of Central South University, Changsha, 410008, Department of Neurology, Xiangya Hospital of Central South University, Changsha, 410008, Department of Neurology, Xiangya Hospital of Central South University, Changsha, 410008



Read Full-Text article