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Serum IL-33 level and IL-33, IL1RL1 gene polymorphisms in asthma and multiple sclerosis patients

Author(s):

Maryam Ahmadi, Farshid Fathi, Saloomeh Fouladi, Fereshteh Alsahebfosul, Mostafa Manian and Nahid Eskandari*  

Abstract:


Background: Asthma is a chronic and complex inflammatory disease of the respiratory tract. Also multiple sclerosis (MS) is a chronic inflammatory demyelinating disease of the central nervous system. Against this background, IL-33 and IL1RL1 play a critical part in autoimmune and inflammatory disorders. Here we explored the IL-33 serum level and 2 potential genetic variants in the IL33 gene and its receptor in Iranian asthma and MS patients.

Methods: This study consisted of asthma (n=140) and MS patients (n=140), and healthy subjects (n=72). Genotyping was carried out in 2 genetic polymorphisms, rs1342326 variant of IL-33 and rs10204137 SNP of IL-33 receptor genes, using High Resolution Melt Real Time PCR based method. The level of serum IL-33 also was measured using enzyme-linked immunosorbent assay method.

Results: The level of IL33 was significantly higher in asthma and MS patients compared to control group (P< 0.001- P<0.001). The frequency distribution of the genotype in rs1342326 in IL-33 gene in patients with asthma, MS and healthy subjects were not significantly different (P>0.05). The frequency distribution of the genotype in rs10204137 in IL-33 gene in patients MS and healthy subjects were significantly different (p = 0.013)

Conclusion: Our findings demonstrated that asthma and MS patients had a higher level of IL-33, which IL-33 receptor genetic polymorphism was associated with MS. Further studies in a larger multicenter setting are needed to explore the value of this marker as a risk stratification biomarker.

Keywords:

Asthma, Multiple Sclerosis, IL-33, IL1RL1, polymorphism, IL-33 serum level

Affiliation:

Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Department of Immunology, school of Medicine, Iran University of Medical Sciences, Tehran, Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan



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