Submit Manuscript  

Article Details


Occurrence of Interleukin-2 (330 G/T) Promoter Polymorphism in ARV associated hepatotoxicity

Author(s):

HariOm Singh*, Nayana Nambiar, Dharmesh Samani and Raman R. Gangakhedkar  

Abstract:


Background: IL-2 cytokine is involved in HIV replication and is also known to cause the hepatic injury. Polymorphisms in IL-2 gene is associated with altered interleukin-2 production.

Method: Hence we assessed the prevalence of IL-2-303G/T polymorphism in 165 HIV patients (34 with and 131without hepatotoxicity) and 155 healthy controls using PCR-RFLP method.

Results: In patients with hepatotoxicity, IL-2-303GT, -303GT+TT genotypes were less prevalent as compared to without hepatotoxicity and healthy controls (29.4% vs. 42.7%, 58.8% vs. 69.5%; 29.4% vs. 40.6%, 58.8% vs. 66.5%, respectively). In patients with hepatotoxicity using tobacco and alcohol, IL-2-303GT,-303TT genotypes were distributed higher as compared to non-users (42.9% vs. 25.9%, OR=8.52, 42.9% vs. 25.9%, OR=9.09, and 28.6% vs. 29.6%, OR=1.63, 42.9% vs. 25.9%, OR=2.93), while IL-2-303TT genotype occurred more often in HIV patients consuming alcohol (34.1% vs. 23.0%). Nevirapine users with hepatotoxicity overrepresented the IL-2-303GT,-303TT genotypes as compared to efaviranz (34.8% vs. 18.2%, OR=4.64, 34.8% vs. 18.2%, OR=3.88). Among nevirapine users, IL-2-303GT genotype was associated with susceptibility to acquisition of hepatotoxicity with borderline significance (OR=4.24, P=0.06). HIV patients using neverapine majorly represented the IL-2-303TT genotype (26.9% vs. 25.0%, OR=2.35) while HIV patients with nevirapine + alcohol usage presented the IL-2 -330TT genotype at a higher frequency (34.2%% vs. 23.5%, OR=1.51). In patients with hepatotoxicity using nevirapine + alcohol the genotype IL-2 -330TT was predominant (60.0% vs. 27.8%, OR=3.16).

Conclusion: Thus, IL-2-303G/T polymorphism did not confer the susceptibility to ARV associated hepatotoxicity. However, IL-2-303G/T polymorphism with nevirapine usage may facilitate the risk for acquisition of ARV associated hepatotoxicity.

Occurrence, of, Interleukin-2, (330, G/T), Promoter, Polymorphism, in, ARV, associated, hepatotoxicity

Affiliation:

Department of Molecular Biology, National AIDS Research Institute Pune- 411026, Department of Molecular Biology, National AIDS Research Institute Pune- 411026, Department of Molecular Biology, National AIDS Research Institute Pune- 411026, Department of Clinical Sciences, National AIDS Research Institute Pune- 411026



Full Text Inquiry