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Isothermal Amplification Methods for the SNP Genotyping

[ Vol. 19 , Issue. 7 ]

Author(s):

Somayeh Heidari Sharafdarkolaee, Pooria Gill*, Majid Motovali-Bashi and Fatemeh Heidari Sharafdarkolaee   Pages 461 - 472 ( 12 )

Abstract:


The demands for genotyping techniques with acceptable precision, accuracy, cost-effectiveness in high throughput formats made driving forces for continuous development of novel technologies. A wide range of mutation detection techniques based on polymerase chain reaction (PCR) have been introduced. The best alternatives were the isothermal amplification technologies that those did not require a thermal cycler. In this review, we aimed to describe the most known isothermal amplification techniques for SNP genotyping.

Keywords:

Mutation detection, SNP, Genotyping, Isothermal amplification, Polymerase chain reaction, LAMP.

Affiliation:

Department of Genetics, Faculty of Sciences, University of Isfahan, Isfahan, Nanomedicine Group, Immunogenetics Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Department of Genetics, Faculty of Sciences, University of Isfahan, Isfahan, Department of biology, Payam Noor University, Mazandaran, Sari



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