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High Glucose Affects the Cytotoxic Potential of Rapamycin, Metformin and Hydrogen Peroxide in Cultured Human Mesenchymal Stem Cells

[ Vol. 19 , Issue. 9 ]

Author(s):

Azam Roohi, Mahin Nikougoftar, Hamed Montazeri, Shadisadat Navabi, Fazel Shokri, Seyed Nasser Ostad and Mohammad Hossein Ghahremani*   Pages 688 - 698 ( 11 )

Abstract:


Background: Oxidative stress and chronic hyperglycemia are two major side effects of type 2 diabetes affecting all cell types including mesenchymal stem cells (MSCs). As a cell therapy choice, understanding the behavior of MSCs will provide crucial information for efficient treatment.

Methods: Placental mesenchymal stem cells were treated with various concentrations of glucose, metformin, rapamycin, and hydrogen peroxide to monitor their viability and cell cycle distribution. Cellular viability was examined via the MTT assay. Cell cycle distribution was studied by propidium iodide staining and apoptosis was determined using Annexin Vpropidium iodide staining and flow cytometry. Involvement of potential signaling pathways was evaluated by Western blotting for activation of Akt, P70S6K, and AMPK.

Results: The results indicated that high glucose augmented cell viability and reduced metformin toxic potential. However, the hydrogen peroxide and rapamycin toxicities were exacerbated.

Conclusion: Our findings suggest that high glucose concentration has a major effect on placental mesenchymal stem cell viability in the presence of rapamycin, metformin and hydrogen peroxide in culture.

Keywords:

Glucose, rapamycin, metformin, hydrogen peroxide, mesenchymal stem cell, cytotoxic potential.

Affiliation:

Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Blood Transfusion Research Center- Higher Institute for Research and Education in Transfusion Medicine- Tehran, School of Pharmacy- International Campus, Iran University of Medical Sciences- Tehran, Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Department of Toxicology-Pharmacology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Department of Toxicology-Pharmacology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran



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