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The Effects of Butyric Acid on the Differentiation, Proliferation, Apoptosis, and Autophagy of IPEC-J2 Cells

Author(s):

Yuan Yang, Jin Huang, Jianzhong Li, Huansheng Yang* and Yulong Yin   Pages 1 - 11 ( 11 )

Abstract:


Butyric acid (BT), a short-chain fatty acid, is the preferred colonocyte energy source. The effects of BT on the differentiation, proliferation, and apoptosis of small intestinal epithelial cells of piglets and its underlying mechanisms are not yet fully elucidated. In this study, we found that 0.2-0.4 mM BT promoted the procine jejuna epitheial cell line (IPEC-J2) cells differentiation. BT at 0.5 mM or higher concentrations significantly impaired cell viability in a dose- and time- dependent manner. In addition, BT at high concentrations inhibited the IPEC-J2 cells proliferation and induced cell cycle arrest in the G2/M phase. Our results demonstrate that BT triggered IPEC-J2 cell apoptosis via the caspase8- caspase3 pathway and was accompanied by excess reactive oxygen species (ROS) and TNF-α production. BT at high concentrations inhibited cell autophagy associated with increased lysosome formation. We found that BT-reduced IPEC-J2 cell viability can be attenuated by p38 MAPK inhibitor SB202190. And SB202190 attenuated BT-increased p38 MAPK target DDIT3 mRNA level and V-ATPase mRNA level that responsible for normal acidy lyososomes. In conclusion, 1) at 0.2-0.4 mM, BT promotes the IPEC-J2 cells differentiation; 2) BT at 0.5 mM or higher concentrations induce cell apoptosis via the p38 MAPK pathway; 3) BT inhibits cells autophagy and promote lysosome formation at high concentrations.

Keywords:

butyrate, p38 MAPK, apoptosis, autophagy, V-ATPase, lysosome

Affiliation:

Hunan International Joint Laboratory of Animal Intestinal Ecology and Health, Nutrition and Human Health Laboratory, School of Life Sciences, Hunan Normal University, Changsha City, Hunan 410081, Hunan International Joint Laboratory of Animal Intestinal Ecology and Health, Nutrition and Human Health Laboratory, School of Life Sciences, Hunan Normal University, Changsha City, Hunan 410081, Hunan International Joint Laboratory of Animal Intestinal Ecology and Health, Nutrition and Human Health Laboratory, School of Life Sciences, Hunan Normal University, Changsha City, Hunan 410081, Hunan International Joint Laboratory of Animal Intestinal Ecology and Health, Nutrition and Human Health Laboratory, School of Life Sciences, Hunan Normal University, Changsha City, Hunan 410081, Hunan International Joint Laboratory of Animal Intestinal Ecology and Health, Nutrition and Human Health Laboratory, School of Life Sciences, Hunan Normal University, Changsha City, Hunan 410081



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