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RPS24c Isoform Facilitates Tumor Angiogenesis Via Promoting the Stability of MVIH in Colorectal Cancer

Author(s):

Yue Wang*, Youjun Wu, Kun Xiao*, Yingjie Zhao, Gang Lv, Shiyan Xu and Fuquan Wu   Pages 1 - 8 ( 8 )

Abstract:


Background: Colorectal cancer (CRC) is the seconding leading cause of death of cancer worldwide, and distant metastasis is responsible for the poor prognosis in patients with advanced-stage CRC. RPS24 (ribosomal protein S24) as a ribosomal protein, multiple transcript variant encoding different isoforms have been found for this gene. Our previous studies have demonstrated that RPS24 is overexpressed in CRC. However, the mechanisms underlying the role of RPS24 in tumor development have not been fully defined.

Methods: Expression of RPS24 isoforms and lncRNA MVIH in CRC tissues and cell lines were quantified by real time PCR or western blotting assay. Endothelial tube formation assay was performed to determine the effect of RPS24 on tumor angiogenesis. The cell viability of HUVEC was determined by MTT assay, and the migration and invasion ability of HUVEC was detected by transwell assy. PGK1 secretion was tested with a specific ELISA kit.

Results: Here we found that RPS24c isoform was a major contributor to tumor angiogenesis, a vital process in tumor growth and metastasis. Real time PCR revealed that RPS24c isoform was high expressed in CRC tissues, while other isoforms are present in both normal and CRC tissues with no statistical difference. And change of RPS24 protein level is mainly due to fluctuation of RPS24c. Furthermore, we observed that silencing RPS24c could decrease angiogenesis by inhibiting tubule formation, HUVEC cell proliferation and migration. Additionally, we investigated the molecular mechanisms and demonstrated that RPS24c mRNA interacted with lncRNA MVIH, the binding-interaction enhanced the stability of each other, thereby activated angiogenesis by inhibiting the secretion of PGK1.

Conclusion: RPS24c facilitates tumor angiogenesis via the RPS24c/MVIH/PGK1 pathway in CRC. RPS24c inhibition may be a novel option for anti-vascular treatment in CRC.

Keywords:

RPS24, Isoform, Colorectal cancer, Angiogenesis, MVIH, PGK1

Affiliation:

Department of General Surgery, The 8th Medical Center of Chinese PLA General Hospital, 17 Heishanhu Road, Beijing, Department of General Surgery, The 8th Medical Center of Chinese PLA General Hospital, 17 Heishanhu Road, Beijing, Department of Pulmonary & Critical Care Medicine, Chinese People’s Liberation Army (PLA) General Hospital, Beijing, Department of General Surgery, The 8th Medical Center of Chinese PLA General Hospital, 17 Heishanhu Road, Beijing, Department of General Surgery, The 8th Medical Center of Chinese PLA General Hospital, 17 Heishanhu Road, Beijing, Department of General Surgery, The 8th Medical Center of Chinese PLA General Hospital, 17 Heishanhu Road, Beijing, Department of General Surgery, The 8th Medical Center of Chinese PLA General Hospital, 17 Heishanhu Road, Beijing



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