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Evaluation of Possible Processing Time Effects on the Global N-Glycosylation Profile of Human Blood Samples

[ Vol. 20 , Issue. 10 ]

Author(s):

Rebeka Torok*, Anna Farkas, Andras Guttman and Gabor Jarvas   Pages 840 - 846 ( 7 )

Abstract:


The utilization of N-glycan profiling recently gained high importance in fundamental biomedical and applied clinical research. However, for the time being, no glycan biomarker has been approved for clinical diagnosis by the regulatory agencies due to the lack of verifications on large patient cohorts and suitable analytical technologies. In this paper, the effect of human blood sample handling was studied prior to N-glycosylation profiling by capillary electrophoresis, coupled with high sensitivity fluorescence detection. Special attention was paid to the preservation of sialylated structures because of their important clinical - biological relevance. Our results suggested that it is adequate to refrigerate and store the collected total blood samples prior to analysis to obtain unbiased results. Furthermore, we report on the good practice of serum sample handling in order to prevent decomposition of the sialylated structures. Our findings may promote procedure standardization and easier clinical translation of diagnostic N-glycosylation profiling in molecular medicinal applications.

Keywords:

Blood sampling, storage temperature, storage time, preanalytical processing, N-glycome, capillary electrophoresis.

Affiliation:

Research Institute of Biomolecular and Chemical Engineering, University of Pannonia, Veszprem, Horvath Csaba Memorial Laboratory of Bioseparation Sciences, Research Center for Molecular Medicine, Doctoral School of Molecular Medicine, Faculty of Medicine, University of Debrecen, Debrecen, Research Institute of Biomolecular and Chemical Engineering, University of Pannonia, Veszprem, Research Institute of Biomolecular and Chemical Engineering, University of Pannonia, Veszprem



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