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Del-1 in Psoriasis Induced the Expression of αvβ3 and α5β1 in Endothelial Cells

Author(s):

Xuping Niu, Qixin Han, Xiaofang Li, Juan Li, Yanmin Liu, Yan Li and Yan Wu*   Pages 1 - 7 ( 7 )

Abstract:


Objective: Psoriasis is a chronic inflammatory skin disease highly depending on angiogenesis. Our prior results showed that the mRNA and protein of Del-1 in dermal mesenchymal stem cells (dMSCs) was up-regulated from psoriasis. Our aim was further to investigate the role of Del-1 from dMSCs in the pathogenesis of psoriasis and confirm the effect of Del-1 on the pathogenesis of psoriasis.

Methods: We conducted an immunohistochemistry experiment to further investigate the expression of Del-1in psoriatic lesions. In addition, dMSCs with over-expressed Del-1 via the lentiviral vector of Del-1 were co-cultured with ECs, and the protein expression of integrins (αvβ3, αvβ5 ,and α5β1) of ECs were detected by western blotting.

Results: This research showed that Del-1 was significantly increased in lesions of patients with psoriasis (p< .05, 9.96 vs. 2.18), and Del-1 from dMSCs successfully induced up-regulation of integrins α5β1 and αvβ3 (all p < .05).

Conclusion: This study demonstrated that Del-1 from dMSCs was involved in the pathogenesis of psoriasis through induced angiogenesis. And Del-1, αvβ3 and α5β1 may be potential new targets for inhibiting angiogenesis in psoriasis.

Keywords:

Psoriasis, Del-1, dermal mesenchymal stem cells (dMSCs), integrin, angiogénesis, western blotting.

Affiliation:

Department of Dermatology, The First Hospital of China Medical University, Shenyang, Department of Dermatology, The First Hospital of China Medical University, Shenyang, Shanxi Key Laboratory of Stem Cells for Immunological Dermatosis, Institute of Dermatology, Taiyuan Central Hospital of Shanxi Medical University, Taiyuan, Shanxi, Shanxi Key Laboratory of Stem Cells for Immunological Dermatosis, Institute of Dermatology, Taiyuan Central Hospital of Shanxi Medical University, Taiyuan, Shanxi, Shanxi Key Laboratory of Stem Cells for Immunological Dermatosis, Institute of Dermatology, Taiyuan Central Hospital of Shanxi Medical University, Taiyuan, Shanxi, No. 1 English Department, School of Fundamental Sciences, China Medical University, Shenyang, Department of Dermatology, The First Hospital of China Medical University, Shenyang



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