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Effects of Myo-inositol Alone and in Combination with Seleno-Lmethionine on Cadmium-Induced Testicular Damage in Mice

[ Vol. 12 , Issue. 4 ]

Author(s):

Salvatore Benvenga, Antonio Micali*, Giovanni Pallio, Roberto Vita, Consuelo Malta, Domenico Puzzolo, Natasha Irrera, Francesco Squadrito, Domenica Altavilla and Letteria Minutoli   Pages 311 - 323 ( 13 )

Abstract:


<P>Background: Cadmium (Cd) impairs gametogenesis and damages the blood-testis barrier. </P><P> Objective: As the primary mechanism of Cd-induced damage is oxidative stress, the effects of two natural antioxidants, myo-inositol (MI) and seleno-L-methionine (Se), were evaluated in mice testes. </P><P> Methods: Eighty-four male C57 BL/6J mice were divided into twelve groups: 0.9% NaCl (vehicle; 1 ml/kg/day i.p.); Se (0.2 mg/kg/day per os); Se (0.4 mg/kg/day per os); MI (360 mg/kg/day per os); MI plus Se (0.2 mg/kg/day); MI plus Se (0.4 mg/kg/day); CdCl2 (2 mg/kg/day i.p.) plus vehicle; CdCl2 plus MI; CdCl2 plus Se (0.2 mg/kg/day); CdCl2 plus Se (0.4 mg/kg/day); CdCl2 plus MI plus Se (0.2 mg/kg/day); and CdCl2 plus MI plus Se (0.4 mg/kg/day). After 14 days, testes were processed for biochemical, structural and immunohistochemical analyses. </P><P> Results: CdCl2 increased iNOS and TNF-&#945; expression and Malondialdehyde (MDA) levels, lowered glutathione (GSH) and testosterone, induced testicular lesions, and almost eliminated claudin-11 immunoreactivity. Se administration at 0.2 or 0.4 mg/kg significantly reduced iNOS and TNF-&#945; expression, maintained GSH, MDA and testosterone levels, structural changes and low claudin-11 immunoreactivity. MI alone or associated with Se at 0.2 or 0.4 mg/kg significantly reduced iNOS and TNF-&#945; expression and MDA levels, increased GSH and testosterone levels, ameliorated structural organization and increased claudin-11 patches number. </P><P> Conclusion: We demonstrated a protective effect of MI, a minor role of Se and an evident positive role of the association between MI and Se on Cd-induced damages of the testis. MI alone or associated with Se might protect testes in subjects exposed to toxicants, at least to those with behavior similar to Cd.</P>

Keywords:

Endocrine disruptors, cadmium, myo-inositol, seleno-L-methionine, testis, spermatogenesis.

Affiliation:

Department of Clinical and Experimental Medicine, University Hospital &#34;G. Martino&#34;, Messina, Department of Biomedical and Dental Sciences and Morphofunctional Imaging, University Hospital &#34;G. Martino&#34;, Messina, Department of Clinical and Experimental Medicine, University Hospital &#34;G. Martino&#34;, Messina, Department of Clinical and Experimental Medicine, University Hospital &#34;G. Martino&#34;, Messina, Department of Biomedical and Dental Sciences and Morphofunctional Imaging, University Hospital &#34;G. Martino&#34;, Messina, Department of Biomedical and Dental Sciences and Morphofunctional Imaging, University Hospital &#34;G. Martino&#34;, Messina, Department of Clinical and Experimental Medicine, University Hospital &#34;G. Martino&#34;, Messina, Department of Clinical and Experimental Medicine, University Hospital &#34;G. Martino&#34;, Messina, Department of Biomedical and Dental Sciences and Morphofunctional Imaging, University Hospital &#34;G. Martino&#34;, Messina, Department of Clinical and Experimental Medicine, University Hospital &#34;G. Martino&#34;, Messina

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