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Apigenin Inhibits Proliferation, Migration, and Invasion of Human Tongue Carcinoma Tca8113 Cells Through Regulating the MAPK Signaling Pathways


Yun-Ze Xuan, Li-Zhi Xu, Cheng-Ri Jin and Kang-Juan Yang*   Pages 1 - 8 ( 8 )


Objective: This study aimed to examine the effects of apigenin (API) on the proliferation, migration, and invasion of human tongue squamous cell carcinoma Tca8113 cells and explore its probable mechanisms.

Methods: After treating Tca8113 cells with API, the cell proliferation, migration, and invasivecapacities were identified by te-trazolium salt colorimetry(MTT) assay, cell scratch test, and Transwell chamber test. Cellular immunofluorescence staining was used to localize mitogen-activated protein kinase 1 (MAPK1) and extracellular regulated protein kinase (ERK) 1/2 proteins. Western blot was used to detect the variations of the related protein expression levels.

Results: ① Through the MTT assay, API significantly inhibited cell proliferation (P<0.01). ②In the cell scratch test, the dis-tance of lateral migration after the API treatment was significantly shorter compared to the control group (P<0.01). ③The inva-sion rate in the lower chamber of the Transwell chamber was lower in the API group (P<0.01). ④ Cellular immunofluores-cence staining presented that the total-MEKK1 was localized in the cytoplasm, p-MEKK1 was localized in the nuclear mem-brane and cytoplasm, and p-ERK1/2 was localized in the cytoplasm and nucleus. ⑤ After API was applied to cells, the expres-sions of p-MEKK1 and p-ERK1/2 proteins significantly reduced (P<0.01).

Conclusion: Apigenin(API) significantly inhibits the proliferation, migration, and invasion of Tca8113 cells and its mecha-nism may be associatedwith the MAPK signaling pathway.


Apigenin(API), Tca8113 cell, mitogen-aetivated protein kinase signaling pathways, cell proliferation


Department of Dentistry, Affiliated Hospital of Yanbian University, Yanji, Jilin 133000, Department of Head and Neck Oncology, Stomatological Hospital, Air Force Medical University,Xi'an,Shanxi 710000, Department of Dentistry, Affiliated Hospital of Yanbian University, Yanji, Jilin 133000, Department of Cell Biology and Medical Genetics, Yanbian University College of Basic Medicine, Yanji,Jilin13300

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