Submit Manuscript  

Article Details


Diet-induced obesity differentially modulates cardiac inflammatory status in the C57 and FVB mouse strains

Author(s):

Fernanda Gaisler-Silva, Carolina Victoria Cruz Junho, Izabelle Fredo-da-Costa, Marcelo Augusto Christoffolete and Marcela Sorelli Carneiro-Ramos*  

Abstract:


Background: Cardiovascular diseases correspond to the highest risk of sudden death worldwide, and obesity is largely related to being an increased risk factor. There is a higher prevalence of arterial hypertension in obese individuals, including the presence of cardiac hypertrophy.

Methods: It is already known the role of toll-like receptors [TLR], mainly 2 and 4 in heart cells, as fundamental to the process of cardiac hypertrophy. Obesity has been studied as an activator of damage-associated molecular patterns [DAMPs], which use the TLR signaling pathway to increase the nuclear factor of inflammation, NF-kB, increasing cytokine expression in heart tissue. It’s already known that FVB/N and C57BL/6 mouse strains have different behaviors in relation to metabolism, but the difference in cardiac tropism and innate immune system modulation is not clear.

Results: The present study aimed to evaluate the contribution of innate immune factors to cardiac hypertrophy induced by an experimental model of obesity comparing two mouse strains: C57BL/6 and FVB/N. Both strains were submitted to a high-fat diet containing 23% protein, 35.5% carbohydrate, and 35.9% fat for 68 days. Hearts were collected, weighed, and submitted to RT-qPCR, and iBoplex analyzed the serum. We observed an increase in heart mass after 68 days in both strains.

Conclusion: This was followed by an increase of -actin only in C57BL/6 while ANF was increased in FVB/N. Gene expression of innate immune components and inflammatory cytokines were only increased in C57BL/6, but not in FVB/N. Based on the results obtained, we verified that C57BL/6 mice had a more robust action of innate immune system than FVB/N.

Keywords:

Cardiac hypertrophy, obesity, high-fat diet, immune response, TLR, C57BL/6, FVB/N

Affiliation:

Centro de Ciências Naturais e Humanas [CCNH]. Universidade Federal do ABC, Santo André, SP, Centro de Ciências Naturais e Humanas [CCNH]. Universidade Federal do ABC, Santo André, SP, Centro de Ciências Naturais e Humanas [CCNH]. Universidade Federal do ABC, Santo André, SP, Centro de Ciências Naturais e Humanas [CCNH]. Universidade Federal do ABC, Santo André, SP, Centro de Ciências Naturais e Humanas [CCNH]. Universidade Federal do ABC, Santo André, SP



Full Text Inquiry