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Silencing of Disease-related Genes by Small Interfering RNAs

[ Vol. 4 , Issue. 5 ]

Author(s):

U. Fuchs, C. Damm-Welk and A. Borkhardt   Pages 507 - 517 ( 11 )

Abstract:


In recent years a new mechanism of posttranscriptional gene silencing has been discovered and named RNA interference. The interference is based on mRNA degradation mediated by small double-stranded RNA molecules approximately 21 nucleotides in length, the so-called short interfering or siRNAs. These molecules are produced from long dsRNAs by Dicer, a dsRNA-specific endonuclease, and cause specific degradation of their mRNA-targets by Watson-Crick base-pairing within a 300 kD multi-enzyme complex named RISC. RNAi is highly conserved between plants and animals of various phyla including mammals. The high sequence-specificity of RNAi makes it a new, promising tool in gene-function analysis as well as in potential therapeutics. In this review the discovery and molecular background of RNAi are summarized and possible fields of application pointed out.

Keywords:

rnai, dicer, risc, ptgs, cancer, treatment strategies

Affiliation:

Pediatric Hematology&Oncology, Children's University Hospital Giessen, Feulgenstrasse12, 35392 Giessen, Germany



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